Backed into favouritism, the Darren Weir trained filly got a little further back than expected in the 1000 metre scamper, but was able to thread her way through the field for Damian Lane.
She slipped along the inside late to snatch a short half head win over Pierro's brilliant daughter Tulip, who lost no admirers with her tenacious effort at her second start.
Limestone clocked 56.88 seconds, which was slightly quicker than the colts division won by Property.
"She's an absolute ripper and a credit to Darren Weir and his team," said Damian Lane.
Weir is not renowned for his training of two year-olds, so Limestone is breaking new ground for the leading trainer.
"She got back a bit further than I thought," said Weir.
"She also had her head on one side coming up the straight, which might be just greenness, I just hope she pulls up well.
"I've never had a two year-old like her before. I'll have to have a think about where we go next, although maybe another run before the Blue Diamond."
A homebred for David Moodie's Hesket Bloodstock, Limestone has won two of three starts earning $176,850 and will progress towards the Group I MRC Blue Diamond Stakes next month.
She is the second foal and second winner from Limerock, a winning Charge Forward half-sister to stakes-winner Warpath.
Limerock has a yearling filly by Al Maher, a weanling colt by Zofffany (IRE) and was covered last spring by Sebring.
Limestone is the third stakes-winner for Darley's triple Group I winner Helmet, who has a big draft of 23 yearlings for the upcoming 2017 Inglis Premier Yearling Sale. Click here to see them.